XE6 Xwnt-5A Hind III CSKA
(Plasmid
#16871)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 16871 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backboneCSKA
- Backbone size w/o insert (bp) 4120
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Vector typeXenopus Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameXwnt-5A
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Alt namexwnt5
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Alt namewnt 5
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SpeciesX. laevis (frog)
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Insert Size (bp)1526
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Entrez Genewnt5a.S (a.k.a. XELAEV_18003033mg, wnt-5A, wnt5a, wnt5a-B, wnt5a-a)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer NA (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Insert: oocyte cDNA from Melton lambda gt10 library.
Notes for Use:
This is an Xwnt-5A Hind III insert under the control of a cytoskeletal actin promoter. See comments for Xwnt-5A Hind RNA regarding initiation met, as well as, Genbank sequence.
Citation: Moon et al., Development 119, 97 (1993)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
XE6 Xwnt-5A Hind III CSKA was a gift from Randall Moon (Addgene plasmid # 16871 ; http://n2t.net/addgene:16871 ; RRID:Addgene_16871) -
For your References section:
Xwnt-5A: a maternal Wnt that affects morphogenetic movements after overexpression in embryos of Xenopus laevis. Moon RT, Campbell RM, Christian JL, McGrew LL, Shih J, Fraser S. Development. 1993 Sep. 119(1):97-111. PubMed 8275867