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Addgene

XE6 Xwnt-5A Hind III CSKA
(Plasmid #16871)

Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 16871 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    CSKA
  • Backbone size w/o insert (bp) 4120
  • Vector type
    Xenopus Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Xwnt-5A
  • Alt name
    xwnt5
  • Alt name
    wnt 5
  • Species
    X. laevis (frog)
  • Insert Size (bp)
    1526
  • Entrez Gene
    wnt5a.S (a.k.a. XELAEV_18003033mg, wnt-5A, wnt5a, wnt5a-B, wnt5a-a)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Insert: oocyte cDNA from Melton lambda gt10 library.

Notes for Use:
This is an Xwnt-5A Hind III insert under the control of a cytoskeletal actin promoter. See comments for Xwnt-5A Hind RNA regarding initiation met, as well as, Genbank sequence.

Citation: Moon et al., Development 119, 97 (1993)

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    XE6 Xwnt-5A Hind III CSKA was a gift from Randall Moon (Addgene plasmid # 16871 ; http://n2t.net/addgene:16871 ; RRID:Addgene_16871)
  • For your References section:

    Xwnt-5A: a maternal Wnt that affects morphogenetic movements after overexpression in embryos of Xenopus laevis. Moon RT, Campbell RM, Christian JL, McGrew LL, Shih J, Fraser S. Development. 1993 Sep. 119(1):97-111. PubMed 8275867