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Addgene

XE7 Xwnt-8
(Plasmid #16869)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 16869 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    SP64T
  • Backbone size w/o insert (bp) 3000
  • Vector type
    Xenopus Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Xwnt-8 RNA
  • Alt name
    xwnt-8
  • Alt name
    wnt-8a
  • Species
    X. laevis (frog)
  • Insert Size (bp)
    3230
  • Entrez Gene
    wnt8a.L (a.k.a. XELAEV_18017109mg, Xwnt8, wnt-8, wnt8, wnt8a, xwnt-8)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Bgl II (destroyed during cloning)
  • 3′ cloning site Bgl II (destroyed during cloning)
  • 5′ sequencing primer SP6
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Insert: EcoR1 insert blunted into Bgl II blunt of SP64T.
Transcription: linearize with Bam H1 and transcribe with SP6.

Plasmid History:
Constructed by Jan Christian on date of 12/90.

Citation: Christian et al., Development 111, 1045 (1991)

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    XE7 Xwnt-8 was a gift from Randall Moon (Addgene plasmid # 16869 ; http://n2t.net/addgene:16869 ; RRID:Addgene_16869)

  • For your References section:

    Xwnt-8, a Xenopus Wnt-1/int-1-related gene responsive to mesoderm-inducing growth factors, may play a role in ventral mesodermal patterning during embryogenesis. Christian JL, McMahon JA, McMahon AP, Moon RT. Development. 1991 Apr. 111(4):1045-55. PubMed 1879349
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