pDONR223 HCoV-NL63 NSP16_stop
(Plasmid
#168681)
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PurposeGateway-compatible Entry vector
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 168681 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 * |
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Backbone
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Vector backbonepDONR223
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Backbone manufacturerRual et al, Genome Res. 14:2128-2135, 2004.
- Backbone size w/o insert (bp) 5005
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Vector typeOther
Growth in Bacteria
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Bacterial Resistance(s)Spectinomycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameNSP16
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SpeciesOther
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Insert Size (bp)903
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Entrez GeneORF1ab (a.k.a. HCNV63gp1)
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer m13-For
- 3′ sequencing primer m13-Rev (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made byFalter-Braun Lab
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pDONR223 HCoV-NL63 NSP16_stop was a gift from Fritz Roth (Addgene plasmid # 168681 ; http://n2t.net/addgene:168681 ; RRID:Addgene_168681) -
For your References section:
A resource of human coronavirus protein-coding sequences in a flexible, multipurpose Gateway Entry clone collection. Weller B, Lin CW, Pogoutse O, Sauer M, Marin-de la Rosa N, Strobel A, Young V, Knapp JJ, Rayhan A, Falter C, Kim DK, Roth FP, Falter-Braun P. G3 (Bethesda). 2023 Jul 5;13(7):jkad105. doi: 10.1093/g3journal/jkad105. 10.1093/g3journal/jkad105 PubMed 37267226