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Addgene

XE8 Xwnt-8 myc
(Plasmid #16868)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 16868 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    SP64T
  • Backbone size w/o insert (bp) 3230
  • Vector type
    Xenopus Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Xwnt-8 myc
  • Alt name
    xwnt-8
  • Alt name
    wnt8a
  • Species
    X. laevis (frog)
  • Insert Size (bp)
    1470
  • Entrez Gene
    wnt8a.L (a.k.a. XELAEV_18017109mg, Xwnt8, wnt-8, wnt8, wnt8a, xwnt-8)
  • Tag / Fusion Protein
    • myc

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Bgl II, EcoR1 (blunt) (destroyed during cloning)
  • 3′ cloning site Bgl II (destroyed during cloning)
  • 5′ sequencing primer SP6
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Insert: myc epitope insert between aa's 338-339.
Insert size: 1.47 Kb.
Transcription: linearize with BamH1 and transcribe with SP6.

Plasmid History:
Constructed by Jan Christian.

Citation: Christian and Moon, Genes and Development 7, 13 (1993)

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    XE8 Xwnt-8 myc was a gift from Randall Moon (Addgene plasmid # 16868 ; http://n2t.net/addgene:16868 ; RRID:Addgene_16868)

  • For your References section:

    Interactions between Xwnt-8 and Spemann organizer signaling pathways generate dorsoventral pattern in the embryonic mesoderm of Xenopus. Christian JL, Moon RT. Genes Dev. 1993 Jan. 7(1):13-28. 10.1101/gad.7.1.13 PubMed 8422982
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