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Addgene

pDONR223 HCoV-NL63 NSP14_stop
(Plasmid #168679)

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Item Catalog # Description Quantity Price (USD)
Plasmid 168679 Standard format: Plasmid sent in bacteria as agar stab 1 $85 *
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Backbone

  • Vector backbone
    pDONR223
  • Backbone manufacturer
    Rual et al, Genome Res. 14:2128-2135, 2004.
  • Backbone size w/o insert (bp) 5005
  • Vector type
    Other

Growth in Bacteria

  • Bacterial Resistance(s)
    Spectinomycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    NSP14
  • Species
    Other
  • Insert Size (bp)
    1554
  • Entrez Gene
    ORF1ab (a.k.a. HCNV63gp1)

Cloning Information

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Falter-Braun Lab

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pDONR223 HCoV-NL63 NSP14_stop was a gift from Fritz Roth (Addgene plasmid # 168679 ; http://n2t.net/addgene:168679 ; RRID:Addgene_168679)

  • For your References section:

    A resource of human coronavirus protein-coding sequences in a flexible, multipurpose Gateway Entry clone collection. Weller B, Lin CW, Pogoutse O, Sauer M, Marin-de la Rosa N, Strobel A, Young V, Knapp JJ, Rayhan A, Falter C, Kim DK, Roth FP, Falter-Braun P. G3 (Bethesda). 2023 Jul 5;13(7):jkad105. doi: 10.1093/g3journal/jkad105. 10.1093/g3journal/jkad105 PubMed 37267226
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