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XE9 Xwnt-8 CSKA
(Plasmid #16866)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 16866 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    CSKA
  • Backbone size w/o insert (bp) 4120
  • Vector type
    Xenopus Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Xwnt-8
  • Alt name
    wnt-8
  • Species
    X. laevis (frog)
  • Insert Size (bp)
    1420
  • Entrez Gene
    wnt8a.L (a.k.a. XELAEV_18017109mg, Xwnt8, wnt-8, wnt8, wnt8a, xwnt-8)
  • Tag / Fusion Protein
    • myc?

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoR1 (unknown if destroyed)
  • 5′ sequencing primer NA
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Insert: EcoR1 fragment of Xwnt-8 cDNA cloned behind Xenopus Cytoskeleton Actin Promoter(1.42 Kb).

Plasmid History:
Constructed by Jan Christian.

Citation: Christian and Moon, Genes and Development 7, 13 (1993)

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    XE9 Xwnt-8 CSKA was a gift from Randall Moon (Addgene plasmid # 16866 ; http://n2t.net/addgene:16866 ; RRID:Addgene_16866)

  • For your References section:

    Interactions between Xwnt-8 and Spemann organizer signaling pathways generate dorsoventral pattern in the embryonic mesoderm of Xenopus. Christian JL, Moon RT. Genes Dev. 1993 Jan. 7(1):13-28. 10.1101/gad.7.1.13 PubMed 8422982
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