XE176 Xenopus Wnt-8-myc-pCDbeta-G
(Plasmid
#16864)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 16864 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepCD-betaG-PS
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Backbone manufacturerAvailable from Addgene (#17120)
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Vector typeXenopus Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameWnt-8
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Alt nameWnt8
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SpeciesX. laevis (frog)
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Entrez Genewnt8a.L (a.k.a. XELAEV_18017109mg, Xwnt8, wnt-8, wnt8, wnt8a, xwnt-8)
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Tag
/ Fusion Protein
- myc (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer n/a (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Xenopus Wnt-8, tagged with a c-myc epitope at the carboxy terminal end, cloned into the SV40 expression vector pCD-PS, but providing Xenopus beta globin 5' and 3' UTRs.
Reference: The insert was published in J. Christian et al., Genes and Development, 1993. This unpublished construct was generated by Julia Yang-Snyder.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
XE176 Xenopus Wnt-8-myc-pCDbeta-G was a gift from Randall Moon (Addgene plasmid # 16864 ; http://n2t.net/addgene:16864 ; RRID:Addgene_16864)