XE177 Xenopus Wnt-8-myc-pcDNA3
(Plasmid
#16863)
-
Depositing Lab
-
Publication
-
Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 16863 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
-
Vector backbonepcDNA3
- Backbone size w/o insert (bp) 5500
-
Vector typeMammalian Expression
-
Selectable markersNeomycin (select with G418)
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert nameWnt-8
-
Alt nameWnt8
-
SpeciesX. laevis (frog)
-
Entrez Genewnt8a.L (a.k.a. XELAEV_18017109mg, Xwnt8, wnt-8, wnt8, wnt8a, xwnt-8)
-
Tag
/ Fusion Protein
- myc (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer T7 (Common Sequencing Primers)
Resource Information
-
Article Citing this Plasmid
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Xenopus Wnt-8, tagged with a c-myc epitope at the carboxy terminal end, cloned into the vector pcDNA3.
Reference: The insert was published in J. Christian et al., Genes and Development, 1993. This unpublished construct was generated by Julia Yang-Snyder.
How to cite this plasmid
( Back to top)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
XE177 Xenopus Wnt-8-myc-pcDNA3 was a gift from Randall Moon (Addgene plasmid # 16863 ; http://n2t.net/addgene:16863 ; RRID:Addgene_16863)