XE11 Xwnt 8b
(Plasmid
#16862)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 16862 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepCS2+
- Backbone size w/o insert (bp) 4095
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Vector typeXenopus Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameXwnt-8b
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Alt namewnt8
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SpeciesX. laevis (frog)
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Insert Size (bp)2900
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Entrez Genewnt8b (a.k.a. wnt-8b, xwnt-8b, xwnt8b)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer NA (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Constructed by Jeff Brown
Notes for Use:
Insert is from pCSKX8/X8b from Jan Christian. It is a full-length Xwnt-8b with an Xwnt-8 signal peptide, Sal 1/Sma 1 double digest follwed by klenow blunt.
Citation: Cui et al., Development 121, 2177
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
XE11 Xwnt 8b was a gift from Randall Moon (Addgene plasmid # 16862 ; http://n2t.net/addgene:16862 ; RRID:Addgene_16862) -
For your References section:
Xwnt-8b: a maternally expressed Xenopus Wnt gene with a potential role in establishing the dorsoventral axis. Cui Y, Brown JD, Moon RT, Christian JL. Development. 1995 Jul. 121(7):2177-86. PubMed 7635061
Map uploaded by the depositor.
