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Addgene

XE12 Xwnt-11
(Plasmid #16861)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 16861 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pT7TS
  • Vector type
    Xenopus Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Xwnt-11
  • Alt name
    xwnt11
  • Alt name
    wnt
  • Species
    X. laevis (frog)
  • Insert Size (bp)
    1000
  • Entrez Gene
    wnt11b.L (a.k.a. XELAEV_18038627mg, WNT11, wnt-11, wnt11b, xwnt11)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Insert: full length coding seq only, by PCR of lambda clones isolated by Purcell.
Insert size: 1 Kb.
Transciption: linearize with Sal1, Xba1, or BamH1 and transcribe with T7.

Plasmid History:
Constructed by Susan Purcell on date of 5/94.

Citation: Du et al., Molec. Cell Bio., 15, 2625 (1995)
Ku and Melton, Development, 119, 1161 (1993)

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    XE12 Xwnt-11 was a gift from Randall Moon (Addgene plasmid # 16861 ; http://n2t.net/addgene:16861 ; RRID:Addgene_16861)

  • For your References section:

    Identification of distinct classes and functional domains of Wnts through expression of wild-type and chimeric proteins in Xenopus embryos. Du SJ, Purcell SM, Christian JL, McGrew LL, Moon RT. Mol Cell Biol. 1995 May;15(5):2625-34. doi: 10.1128/MCB.15.5.2625. 10.1128/MCB.15.5.2625 PubMed 7739543
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