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Addgene

XE46 dnWnt-1
(Plasmid #16860)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 16860 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCS2+
  • Backbone size w/o insert (bp) 4095
  • Vector type
    Xenopus Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    dnWnt-1
  • Alt name
    wnt-1
  • Alt name
    wnt1
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    915
  • Mutation
    truncated gene
  • Entrez Gene
    Wnt1 (a.k.a. Int-1, Wnt-1, sw, swaying)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoR1 (not destroyed)
  • 3′ cloning site EcoR1 (not destroyed)
  • 5′ sequencing primer SP6
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Plasmid History:
Constructed by Jeff Brown on date of 8/8/95.

Transcription: For sense, linearize with Asp718 (BMB) and transcribe with SP6.

PCR amplification from clone 286 (McMahon and Moon). Also referred to as int-1 spnfc or int-1 spnfcD. The Wnt-1 gene is truncated after the SPNFC peptide sequence.

Ref: Hoppler et al., Genes & Development, 10:2805-2817, 1996.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    XE46 dnWnt-1 was a gift from Randall Moon (Addgene plasmid # 16860 ; http://n2t.net/addgene:16860 ; RRID:Addgene_16860)

  • For your References section:

    Expression of a dominant-negative Wnt blocks induction of MyoD in Xenopus embryos. Hoppler S, Brown JD, Moon RT. Genes Dev. 1996 Nov 1. 10(21):2805-17. 10.1101/gad.10.21.2805 PubMed 8946920
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