XE133 dnWnt-1 myc CS2+
(Plasmid
#16859)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 16859 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepCS2P+
- Backbone size w/o insert (bp) 5010
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Vector typeXenopus Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namednWnt-1 myc CS2+
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Alt namewnt1
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SpeciesM. musculus (mouse)
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MutationWnt-1 gene is truncated after the SPNFC peptide sequence
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Entrez GeneWnt1 (a.k.a. Int-1, Wnt-1, sw, swaying)
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Tag
/ Fusion Protein
- myc
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer SP6 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Transcription: For sense, linearize with Asp718 (BMB) and transcribe with.
SP6. PCR amplified from myc tagged mouse Wnt-1 (McMahon and Moon). Also.
referred to as int-1 spnfc or int-1 spnfcD in notebook #3. The Wnt-1 gene.
is truncated acter the SPNFC peptide sequence.
Notes for Use:
Constructed by J. Brown.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
XE133 dnWnt-1 myc CS2+ was a gift from Randall Moon (Addgene plasmid # 16859 ; http://n2t.net/addgene:16859 ; RRID:Addgene_16859)