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Addgene

pSP64T-dnWnt-11
(Plasmid #16857)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 16857 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pSP64T
  • Backbone manufacturer
    Available at Addgene (#15030)
  • Backbone size w/o insert (bp) 3300
  • Vector type
    Xenopus Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    dnWnt-11
  • Alt name
    Wnt11
  • Alt name
    Xwnt11
  • Species
    X. laevis (frog)
  • Insert Size (bp)
    850
  • Mutation
    amino acids 1-282. Dominant Negative.
  • Entrez Gene
    wnt11b.L (a.k.a. XELAEV_18038627mg, WNT11, wnt-11, wnt11b, xwnt11)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XhoI (unknown if destroyed)
  • 3′ cloning site BglII (unknown if destroyed)
  • 5′ sequencing primer SP6
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

A C-terminally truncated dominant-negative Xwnt11 construct (comprising amino acids 1-282) was generated according to Hoppler
et al. (1996). PCR was carried out using pGEM-X9 (Ku and Melton, 1993) as a template and the primers 5′-CCCCCTCGAGAGTACCAATGGCTCCGACCCG-3′ and 5′-TTGGAGATCTTTCAGCAGTAGTCAGGGGAAC-3′. The PCR product obtained was cloned into the XhoI and BglII sites of pSP64TXB.

For injection, linearize with BamH1 and transcribe with SP6.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSP64T-dnWnt-11 was a gift from Jim Smith (Addgene plasmid # 16857 ; http://n2t.net/addgene:16857 ; RRID:Addgene_16857)

  • For your References section:

    Xwnt11 is a target of Xenopus Brachyury: regulation of gastrulation movements via Dishevelled, but not through the canonical Wnt pathway. Tada M, Smith JC. Development. 2000 May . 127(10):2227-38. PubMed 10769246
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