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XE169 mouse Wnt-1-myc-pGEM4
(Plasmid #16855)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 16855 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pGEM4
  • Backbone size w/o insert (bp) 2746
  • Vector type
    Xenopus Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Wnt-1-myc
  • Alt name
    Wnt-1
  • Species
    M. musculus (mouse)
  • GenBank ID
    NM_021279.4
  • Entrez Gene
    Wnt1 (a.k.a. Int-1, Wnt-1, sw, swaying)
  • Tag / Fusion Protein
    • Myc

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)
  • 3′ cloning site unknown (unknown if destroyed)
  • 5′ sequencing primer M13-Rev
  • 3′ sequencing primer M13-F20
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Description: Full coding sequence of mouse Wnt-1 (int-1 in early literature), with one internal c-myc epitope. Protein can be immunoprecipitated, recognized in whole mount immunostaining, and detected by western blot, using the anti-c-myc antibody 9E-10.

Species of Sequence Origin: mouse.

Notes for Use: Linearize with Eco R1 and transcribe with T7 to make RNA.

Reference: This is the clone published as p284 in AP McMahon and RT Moon, Cell 58: 1075-1084 (1989).

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    XE169 mouse Wnt-1-myc-pGEM4 was a gift from Randall Moon (Addgene plasmid # 16855 ; http://n2t.net/addgene:16855 ; RRID:Addgene_16855)

  • For your References section:

    Ectopic expression of the proto-oncogene int-1 in Xenopus embryos leads to duplication of the embryonic axis. McMahon AP, Moon RT. Cell. 1989 Sep 22;58(6):1075-84. doi: 10.1016/0092-8674(89)90506-0. 10.1016/0092-8674(89)90506-0 PubMed 2673541
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