XE170 mouse Wnt-1-myc-pcDNA3
(Plasmid #16854)

• Depositing Lab: Randall Moon
• Publication: Xenopus Expression Plasmids (unpublished)

Backbone

• Vector backbone: pcDNA3
• Backbone size w/o insert (bp): 5446
• Vector type: Mammalian Expression ; Xenopus Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Wnt1
• Species: M. musculus (mouse)
• Entrez Gene: Wnt1 (a.k.a. Int-1, Wnt-1, sw, swaying)
• Tag / Fusion Protein:
  • myc (internal)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: unknown (unknown if destroyed)
• 3′ cloning site: unknown (unknown if destroyed)
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: BGH-Rev

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Description: Full coding sequence of mouse Wnt-1 (int-1 in early literature), with one internal c-myc epitope, in the Stratagene vector, pcDNA3. Protein can be immunoprecipitated, recognized in whole mount immunostaining, and detected by western blot, using the anti-c-myc antibody 9E-10.

Species of Sequence Origin: mouse.

Notes for Use: Uncharacterized.

Reference: The insert was published as p284 in AP McMahon and RT Moon, Cell 58: 1075-1084 (1989). This unpublished version was generated by Julia Yang-Snyder.