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XE172 antisense mouse Wnt-1-myc-pCDbeta-G
(Plasmid #16852)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 16852 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCD-betaG-PS
  • Vector type
    Xenopus Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    antisense Wnt-1
  • Species
    M. musculus (mouse)
  • Entrez Gene
    Wnt1 (a.k.a. Int-1, Wnt-1, sw, swaying)
  • Tag / Fusion Protein
    • internal myc tag

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)
  • 3′ cloning site unknown (unknown if destroyed)
  • 5′ sequencing primer SV40pro-F
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Description: Antisense orientation of full coding sequence of mouse Wnt-1 (int-1 in early literature), with one internal c-myc epitope, cloned into the SV40 expression vector pCD-PS, but providing Xenopus beta globin 5' and 3' UTRs.

Species of Sequence Origin: mouse.

Notes for Use: Uncharacterized.

Reference: The insert was published as p284 in AP McMahon and RT Moon, Cell 58: 1075-1084 (1989). This unpublished construct was generated by Julia Yang-Snyder.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    XE172 antisense mouse Wnt-1-myc-pCDbeta-G was a gift from Randall Moon (Addgene plasmid # 16852 ; http://n2t.net/addgene:16852 ; RRID:Addgene_16852)
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