p-spTorA-GFP-H6C
(Plasmid #168517)

• Purpose: GFP with the signal peptide of TorA (spTorA) in pBAD24. Includes a C-terminal HHHHHHC (6xHisC) extension.
• Depositing Lab: Siegfried Musser
• Publication: Bageshwar et al PLoS One. 2021 Sep 9;16(9):e0256715. doi: 10.1371/journal.pone.0256715. eCollection 2021.

Backbone

• Vector backbone: pBAD24
• Backbone manufacturer: Beckwith Lab
• Backbone size w/o insert (bp): 4542
• Total vector size (bp): 5386
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: spTorA-GFP-H6C
• Species: Escherichia coli and Aequorea victoria
• Insert Size (bp): 873
• Mutation: The mut3 GFP variant with 5 additional mutations (Q80R, S147H, M153T, V163A, S202D). There are also 3 silent mutations at G65 (GGT instead of GGG), Q69 (CAA instead of CAG) and P75 (CCA instead of CCG).
• GenBank ID: CAD6011594.1 AAB18957.1
• Promoter: araBAD
• Tags / Fusion Proteins:
  • spTorA (signal peptide of E. coli TorA) (N terminal on insert)
  • 6xHisC tag (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: PstI (not destroyed)
• 5′ sequencing primer: pBAD forward primer
• 3′ sequencing primer: pBAD reverse primer

Resource Information

• Supplemental Documents:
  • p-spTorA-GFP-H6C.gbk

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Plasmid was originally reported as pTorA-GFP in Bageshwar & Musser 2007 J. Cell Biol., 179:87-99.