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p-preSufI(IAC)
(Plasmid #168516)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 168516 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET25b
  • Backbone manufacturer
    Novagen (EMD Millipore)
  • Backbone size w/o insert (bp) 5547
  • Total vector size (bp) 6834
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    pre-SufI(IAC)
  • Species
    Escherichia coli
  • Insert Size (bp)
    1412
  • Mutation
    C17I and C295A
  • GenBank ID
    CAD5996880.1
  • Promoter T7
  • Tags / Fusion Proteins
    • HSV tag (C terminal on insert)
    • 6xHisC tag (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer T7 Fwd
  • 3′ sequencing primer T7 Terminator
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Plasmid was originally reported as pSufI-IAC in Bageshwar et al. 2009 Mol. Microbiol., 74:209-226.

This plasmid has 6 silent mutations: L39L (CTG to CTA); P131P (CCT to CCC); L273L (TTG to CTG); G288G (GGT to GGC); A295A (GCA to GCG); and S313S (AGC to AGT).

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    p-preSufI(IAC) was a gift from Siegfried Musser (Addgene plasmid # 168516 ; http://n2t.net/addgene:168516 ; RRID:Addgene_168516)

  • For your References section:

    Influence of the TorD signal peptide chaperone on Tat-dependent protein translocation. Bageshwar UK, DattaGupta A, Musser SM. PLoS One. 2021 Sep 9;16(9):e0256715. doi: 10.1371/journal.pone.0256715. eCollection 2021. 10.1371/journal.pone.0256715 PubMed 34499687
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