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Addgene

pCAG mito-mEos2
(Plasmid #168515)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 168515 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pCAG
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    mito-mEos2
  • Species
    Synthetic
  • Promoter CAG
  • Tag / Fusion Protein
    • mEos2 (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCAG mito-mEos2 was a gift from Franck Polleux (Addgene plasmid # 168515 ; http://n2t.net/addgene:168515 ; RRID:Addgene_168515)
  • For your References section:

    MFF-dependent mitochondrial fission regulates presynaptic release and axon branching by limiting axonal mitochondria size. Lewis TL Jr, Kwon SK, Lee A, Shaw R, Polleux F. Nat Commun. 2018 Nov 27;9(1):5008. doi: 10.1038/s41467-018-07416-2. 10.1038/s41467-018-07416-2 PubMed 30479337