pCAG mEmerald-LAMP1
(Plasmid
#168513)
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PurposeExpression of rat LAMP1 fused with mEmerald to perform live tracking of lysosomes
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 168513 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepCAG
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameLAMP1
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SpeciesR. norvegicus (rat)
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Entrez GeneLamp1 (a.k.a. LGP120)
- Promoter CAG
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Tag
/ Fusion Protein
- mEmerald (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Unknown (unknown if destroyed)
- 3′ cloning site Unknown (unknown if destroyed)
- 5′ sequencing primer NA (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCAG mEmerald-LAMP1 was a gift from Franck Polleux (Addgene plasmid # 168513 ; http://n2t.net/addgene:168513 ; RRID:Addgene_168513) -
For your References section:
Terminal axon branching is regulated by the LKB1-NUAK1 kinase pathway via presynaptic mitochondrial capture. Courchet J, Lewis TL Jr, Lee S, Courchet V, Liou DY, Aizawa S, Polleux F. Cell. 2013 Jun 20;153(7):1510-25. doi: 10.1016/j.cell.2013.05.021. 10.1016/j.cell.2013.05.021 PubMed 23791179