pCAG-Apex2-Venus-CAAX
(Plasmid
#168511)
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PurposeExpression of Apex2 fused to Venus-CAAX for targeting to plasma membrane. Useful for correlated light-eletron microscopy experiments in neurons.
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 168511 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepCAG
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameApex2
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SpeciesGlycine max (Soybean)
- Promoter CAG
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Tag
/ Fusion Protein
- Venus-CAAX (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Unknown (unknown if destroyed)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCAG-Apex2-Venus-CAAX was a gift from Franck Polleux (Addgene plasmid # 168511 ; http://n2t.net/addgene:168511 ; RRID:Addgene_168511) -
For your References section:
Correlated Light-Serial Scanning Electron Microscopy (CoLSSEM) for ultrastructural visualization of single neurons in vivo. Hirabayashi Y, Tapia JC, Polleux F. Sci Rep. 2018 Sep 27;8(1):14491. doi: 10.1038/s41598-018-32820-5. 10.1038/s41598-018-32820-5 PubMed 30262876
