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Addgene

pCAG-Synapto-pHluorin
(Plasmid #168510)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 168510 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCAG
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Synaptophysin-pHluorin
  • Species
    M. musculus (mouse)
  • Entrez Gene
    Syp (a.k.a. A230093K24Rik, Syn, p38)
  • Promoter CAG
  • Tag / Fusion Protein
    • pHluorin (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCAG-Synapto-pHluorin was a gift from Franck Polleux (Addgene plasmid # 168510 ; http://n2t.net/addgene:168510 ; RRID:Addgene_168510)

  • For your References section:

    LKB1 Regulates Mitochondria-Dependent Presynaptic Calcium Clearance and Neurotransmitter Release Properties at Excitatory Synapses along Cortical Axons. Kwon SK, Sando R 3rd, Lewis TL, Hirabayashi Y, Maximov A, Polleux F. PLoS Biol. 2016 Jul 18;14(7):e1002516. doi: 10.1371/journal.pbio.1002516. eCollection 2016 Jul. PBIOLOGY-D-16-00343 [pii] PubMed 27429220
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