XE173 mouse Wnt-1-myc-pCS2+
(Plasmid
#16851)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 16851 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepCS2+
- Backbone size w/o insert (bp) 4100
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Vector typeXenopus Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameWnt-1
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Alt nameWnt1
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Alt nameint-1
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SpeciesM. musculus (mouse)
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Entrez GeneWnt1 (a.k.a. Int-1, Wnt-1, sw, swaying)
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Tag
/ Fusion Protein
- myc
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer SP6 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Full coding sequence of mouse Wnt-1 (int-1 in early literature), with one internal c-myc epitope, cloned into the vector pCS2+
Reference: The insert was published as p284 in AP McMahon and RT Moon, Cell 58: 1075-1084 (1989). This unpublished construct was generated by Julia Yang-Snyder.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
XE173 mouse Wnt-1-myc-pCS2+ was a gift from Randall Moon (Addgene plasmid # 16851 ; http://n2t.net/addgene:16851 ; RRID:Addgene_16851)