pCAG mitoYFP
(Plasmid
#168508)
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PurposeExpression of mito-EYFP (mitochondrial matrix targeted EYFP) for visualization of mitochondrial dynamics
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 168508 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepCAG
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namemitoYFP
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SpeciesSynthetic
- Promoter CAG
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Tag
/ Fusion Protein
- EYFP (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Unknown (unknown if destroyed)
Resource Information
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Supplemental Documents
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCAG mitoYFP was a gift from Franck Polleux (Addgene plasmid # 168508 ; http://n2t.net/addgene:168508 ; RRID:Addgene_168508) -
For your References section:
Progressive Decrease of Mitochondrial Motility during Maturation of Cortical Axons In Vitro and In Vivo. Lewis TL Jr, Turi GF, Kwon SK, Losonczy A, Polleux F. Curr Biol. 2016 Oct 10;26(19):2602-2608. doi: 10.1016/j.cub.2016.07.064. Epub 2016 Sep 15. 10.1016/j.cub.2016.07.064 PubMed 27641765
