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Addgene

pCIG2-Flag-mNuak1 WT
(Plasmid #168507)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 168507 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCIG2
  • Vector type
    Mammalian Expression
  • Selectable markers
    IRES-EGFP

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Nuak1
  • Species
    M. musculus (mouse)
  • Entrez Gene
    Nuak1 (a.k.a. Omphk1, AU014801, AW494241, MGC90816, B230104P22Rik)
  • Promoter CAG
  • Tag / Fusion Protein
    • Flag (N terminal on insert)

Cloning Information

  • Cloning method Ligation Independent Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCIG2-Flag-mNuak1 WT was a gift from Franck Polleux (Addgene plasmid # 168507 ; http://n2t.net/addgene:168507 ; RRID:Addgene_168507)

  • For your References section:

    Terminal axon branching is regulated by the LKB1-NUAK1 kinase pathway via presynaptic mitochondrial capture. Courchet J, Lewis TL Jr, Lee S, Courchet V, Liou DY, Aizawa S, Polleux F. Cell. 2013 Jun 20;153(7):1510-25. doi: 10.1016/j.cell.2013.05.021. 10.1016/j.cell.2013.05.021 PubMed 23791179
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