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Addgene

pCAG-SRGAP3FBAR-EGFP
(Plasmid #168506)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 168506 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCAG
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    SRGAP3-FBAR
  • Species
    H. sapiens (human)
  • Mutation
    aa 1-492
  • Entrez Gene
    SRGAP3 (a.k.a. ARHGAP14, MEGAP, SRGAP2, WRP)
  • Promoter CAG
  • Tag / Fusion Protein
    • EGFP (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCAG-SRGAP3FBAR-EGFP was a gift from Franck Polleux (Addgene plasmid # 168506 ; http://n2t.net/addgene:168506 ; RRID:Addgene_168506)

  • For your References section:

    The F-BAR domains from srGAP1, srGAP2 and srGAP3 regulate membrane deformation differently. Coutinho-Budd J, Ghukasyan V, Zylka MJ, Polleux F. J Cell Sci. 2012 Jul 15;125(Pt 14):3390-401. doi: 10.1242/jcs.098962. Epub 2012 Mar 30. 10.1242/jcs.098962 PubMed 22467852
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