pCAG-SRGAP3-EGFP
(Plasmid
#168504)
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PurposeExpression of full length SRGAP3 with a C-terminal EGFP fusion
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 168504 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepCAG
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameSRGAP3
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SpeciesH. sapiens (human)
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Entrez GeneSRGAP3 (a.k.a. ARHGAP14, MEGAP, SRGAP2, WRP)
- Promoter CAG
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Tag
/ Fusion Protein
- EGFP (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Unknown (unknown if destroyed)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCAG-SRGAP3-EGFP was a gift from Franck Polleux (Addgene plasmid # 168504 ; http://n2t.net/addgene:168504 ; RRID:Addgene_168504) -
For your References section:
The F-BAR domains from srGAP1, srGAP2 and srGAP3 regulate membrane deformation differently. Coutinho-Budd J, Ghukasyan V, Zylka MJ, Polleux F. J Cell Sci. 2012 Jul 15;125(Pt 14):3390-401. doi: 10.1242/jcs.098962. Epub 2012 Mar 30. 10.1242/jcs.098962 PubMed 22467852