pCAG-SRGAP2A-FBAR-mRFP
(Plasmid
#168502)
-
PurposeExpression of the F-BAR domain (aa1-501) of SRGAP2 with a C-terminal mRFP fusion.
-
Depositing Lab
-
Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 168502 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
-
Vector backbonepCAG
-
Vector typeMammalian Expression
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert nameSRGAP2A-FBAR
-
SpeciesH. sapiens (human)
-
Entrez GeneSRGAP2 (a.k.a. ARHGAP34, FNBP2, SRGAP2A, SRGAP3)
- Promoter CAG
-
Tag
/ Fusion Protein
- mRFP (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Unknown (unknown if destroyed)
Resource Information
-
Supplemental Documents
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
( Back to top)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pCAG-SRGAP2A-FBAR-mRFP was a gift from Franck Polleux (Addgene plasmid # 168502 ; http://n2t.net/addgene:168502 ; RRID:Addgene_168502) -
For your References section:
The F-BAR domain of srGAP2 induces membrane protrusions required for neuronal migration and morphogenesis. Guerrier S, Coutinho-Budd J, Sassa T, Gresset A, Jordan NV, Chen K, Jin WL, Frost A, Polleux F. Cell. 2009 Sep 4;138(5):990-1004. doi: 10.1016/j.cell.2009.06.047. 10.1016/j.cell.2009.06.047 PubMed 19737524
