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XE220 mouse Wnt-1-pCS2P+
(Plasmid #16850)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 16850 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCS2P+
  • Backbone size w/o insert (bp) 4100
  • Vector type
    Xenopus Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Wnt-1
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    1500
  • Entrez Gene
    Wnt1 (a.k.a. Int-1, Wnt-1, sw, swaying)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Stu1 (destroyed during cloning)
  • 3′ cloning site Xba1 (destroyed during cloning)
  • 5′ sequencing primer NA
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Description: Full coding sequence of mouse Wnt-1, untagged. ~1.5kb insert isolated from Ajamete Kaykas's 2294 mWnt1 plasmid, Not1filled/Spe1 fragment cloned into pCS2P+ Stu1/Xba1.

Notes for Use: For in vitro transcription linearize with Asp718 and transcribe with SP6.

Reference: Clone made by Gilbert Weidinger, Moon lab.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    XE220 mouse Wnt-1-pCS2P+ was a gift from Randall Moon (Addgene plasmid # 16850 ; http://n2t.net/addgene:16850 ; RRID:Addgene_16850)
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