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pCDNA3_shRNA resistant PKA-Cα(E12,14K)-mEGFP
(Plasmid #168491)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 168491 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3
  • Backbone size w/o insert (bp) 5400
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    the E12,14K mutant of the mouse PKA catalytic subunit α C-terminally tagged by mEGFP. Resistant to shRNA knock-down.

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCDNA3_shRNA resistant PKA-Cα(E12,14K)-mEGFP was a gift from Haining Zhong (Addgene plasmid # 168491 ; http://n2t.net/addgene:168491 ; RRID:Addgene_168491)

  • For your References section:

    Myristoylation alone is sufficient for PKA catalytic subunits to associate with the plasma membrane to regulate neuronal functions. Xiong WH, Qin M, Zhong H. Proc Natl Acad Sci U S A. 2021 Apr 13;118(15). pii: 2021658118. doi: 10.1073/pnas.2021658118. 10.1073/pnas.2021658118 PubMed 33876760
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