pCDNA3_PKA-CαN (G2A)-mVenus
(Plasmid
#168482)
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PurposeMammalian expression of the G2A mutant of the N-terminal 47 residues of mouse PKA catalytic subunit α C-terminally tagged by monomeric Venus.
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 168482 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepcDNA3
- Backbone size w/o insert (bp) 5400
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namethe G2A mutant of the N-terminal 47 residues of mouse PKA catalytic subunit α C-terminally tagged by monomeric Venus
Resource Information
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Supplemental Documents
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A portion of this plasmid was derived from a plasmid made byG. Stan McKnight, University of Washington
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCDNA3_PKA-CαN (G2A)-mVenus was a gift from Haining Zhong (Addgene plasmid # 168482 ; http://n2t.net/addgene:168482 ; RRID:Addgene_168482) -
For your References section:
Myristoylation alone is sufficient for PKA catalytic subunits to associate with the plasma membrane to regulate neuronal functions. Xiong WH, Qin M, Zhong H. Proc Natl Acad Sci U S A. 2021 Apr 13;118(15). pii: 2021658118. doi: 10.1073/pnas.2021658118. 10.1073/pnas.2021658118 PubMed 33876760