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Addgene

XE28 XBC 40
(Plasmid #16842)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 16842 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCS2+MT
  • Backbone size w/o insert (bp) 4300
  • Vector type
    Xenopus Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    beta-catenin-myc
  • Alt name
    beta catenin
  • Species
    X. laevis (frog)
  • Insert Size (bp)
    2300
  • Entrez Gene
    ctnnb1.L (a.k.a. XELAEV_18031149mg, B-catenin, beta-catenin, ctnnb, ctnnb1, ctnnb1-a, ctnnb1-b)
  • Tag / Fusion Protein
    • 6x myc (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Cla1 (unknown if destroyed)
  • 3′ cloning site Cla1 (unknown if destroyed)
  • 5′ sequencing primer SP6
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Plasmid History:
Constructed by David on date of 2/9/95 in book XVI p.106.
Notes for Use:
Full-length Xenopus beta-catenin reconstructed by taking the Cla-SphI vector fragment from XBC20 and the SphI-Cla sequences from XBC24 (encoding the C-terminus of beta-catenin). This corrects possible problems with the C-terminus os XBC20. To make RNA, cut with NotI and transcribe with SP6.

Ref: Yost et al. Genes and Development 10:1443-1454, 1996.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    XE28 XBC 40 was a gift from Randall Moon (Addgene plasmid # 16842 ; http://n2t.net/addgene:16842 ; RRID:Addgene_16842)

  • For your References section:

    The axis-inducing activity, stability, and subcellular distribution of beta-catenin is regulated in Xenopus embryos by glycogen synthase kinase 3. Yost C, Torres M, Miller JR, Huang E, Kimelman D, Moon RT. Genes Dev. 1996 Jun 15. 10(12):1443-54. 10.1101/gad.10.12.1443 PubMed 8666229
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