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Addgene

XE49 pt Beta-catenin-myc
(Plasmid #16840)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 16840 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcS2+MT
  • Backbone size w/o insert (bp) 4352
  • Vector type
    Xenopus Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    pt beta-catenin-myc
  • Alt name
    beta catenin
  • Species
    X. laevis (frog)
  • Mutation
    four point mutations introduced into the GSK-3 phosphorylation site
  • Entrez Gene
    ctnnb1.L (a.k.a. XELAEV_18031149mg, B-catenin, beta-catenin, ctnnb, ctnnb1, ctnnb1-a, ctnnb1-b)
  • Tag / Fusion Protein
    • 6x myc (C terminal on backbone)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

This Xenopus Beta-catenin has four point mutations introduced into the GSK-3 phosphorylation site as shown.
The construct is derived from construct XE-28 and is linearized, transcribed, and used as described there, and in the original citation.

Ref: Yost et al., Genes & Development, 10:1443-1454, 1996.

four mutations are amino acids S33A, S37A, T41A, S45A

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    XE49 pt Beta-catenin-myc was a gift from Randall Moon (Addgene plasmid # 16840 ; http://n2t.net/addgene:16840 ; RRID:Addgene_16840)

  • For your References section:

    The axis-inducing activity, stability, and subcellular distribution of beta-catenin is regulated in Xenopus embryos by glycogen synthase kinase 3. Yost C, Torres M, Miller JR, Huang E, Kimelman D, Moon RT. Genes Dev. 1996 Jun 15. 10(12):1443-54. 10.1101/gad.10.12.1443 PubMed 8666229
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