XE71 NES Beta-catenin GFP in CS2+
(Plasmid
#16837)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 16837 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepCS2+
- Backbone size w/o insert (bp) 4095
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Vector typeXenopus Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameNES beta-catenin GFP
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Alt nameBeta catenin
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SpeciesX. laevis (frog)
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Entrez Genectnnb1.L (a.k.a. XELAEV_18031149mg, B-catenin, beta-catenin, ctnnb, ctnnb1, ctnnb1-a, ctnnb1-b)
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Tag
/ Fusion Protein
- GFP (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer SP6
- 3′ sequencing primer T7 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
NES of rabbit pkI at NH2 terminus of Beta-catenin GFP.
Linearize with Not 1 and transcribe with SP6.
Plasmid History:
Constructed by Jeffery Miller.
Species of Sequence Origin: Xenopus laevis.
Reference: Miller and Moon, 1997, J. Cell Biology 139:229-244.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
XE71 NES Beta-catenin GFP in CS2+ was a gift from Randall Moon (Addgene plasmid # 16837 ; http://n2t.net/addgene:16837 ; RRID:Addgene_16837) -
For your References section:
Analysis of the signaling activities of localization mutants of beta-catenin during axis specification in Xenopus. Miller JR, Moon RT. J Cell Biol. 1997 Oct 6. 139(1):229-43. 10.1083/jcb.139.1.229 PubMed 9314542
Map uploaded by the depositor.
