XE85 Delta Beta-catenin in GFP
(Plasmid #16835)

• Depositing Lab: Randall Moon
• Publication: Xenopus Expression Plasmids (unpublished)

Backbone

• Vector backbone: NA
• Backbone size w/o insert (bp): 6647
• Vector type: Xenopus Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Delta beta-catenin GFP
• Alt name: beta catenin
• Species: X. laevis (frog)
• Mutation: These PCR fragments have the first 138 bp after the AUG removed to eliminate a possible GSK-3 sequence. A new AUG has been inserted just after the Bam site. The mutant GFP of Hiem et al., inserted into Cla I site, followed by a STOP codon
• Entrez Gene: ctnnb1.L (a.k.a. XELAEV_18031149mg, B-catenin, beta-catenin, ctnnb, ctnnb1, ctnnb1-a, ctnnb1-b)
• Tag / Fusion Protein:
  • GFP (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: SP6
• 3′ sequencing primer: T7

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

To make RNA, cut with Not1 and transcribe with SP6.
Plasmid History:
Constructed by Randall Moon.
Note For Use.
Xenopus beta-catenin PCR amplified from st. 19 RNA. These PCR fragments have the first 138 bp after the AUG removed to eliminate a possible GSK-3 sequence. A new AUG has been inserted just after the Bam site. The mutant GFP of Hiem et al., inserted into Cla I site, followed by a STOP codon. This is no myc epitope.
Reference: Moon, 1997, unpublished.