pVDL9.3
(Plasmid #168299)

• Purpose: Secretion of polypeptides (nanobodies) in E. coli fused to C-terminal secretion signal of HlyA
• Depositing Lab: Víctor de Lorenzo
• Publication: Tzschaschel et al Nat Biotechnol. 1996 Jun;14(6):765-9. doi: 10.1038/nbt0696-765.

Backbone

• Vector backbone: pHSG575
• Backbone manufacturer: Tamotsu Hashimoto-Gotoh
• Backbone size w/o insert (bp): 3600
• Total vector size (bp): 7400
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Chloramphenicol, 25 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: Segment of the hly operon, spanning the C-terminal part of hlyA, hlyB, and hlyD
• Species: Synthetic
• Insert Size (bp): 3739
• Promoter: LacI-Plac

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: N/A (unknown if destroyed)
• 3′ cloning site: N/A (unknown if destroyed)
• 5′ sequencing primer: N/A
• 3′ sequencing primer: N/A

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry