CSAC-Bmal1
(Plasmid #168296)

• Purpose: Expresses sgRNA in mammalian cells
• Depositing Lab: Han Kyoung Choe
• Publication: Kim et al Sci Rep. 2021 Jan 28;11(1):2575. doi: 10.1038/s41598-021-82287-0.

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Backbone

• Vector backbone: pAAV-hU6-gRNA-hSyn-mCherry-KASH
• Backbone size w/o insert (bp): 5294
• Total vector size (bp): 5653
• Vector type: AAV

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: hU6-sgBmal1-1-hU6-sgBmal1-3
• Alt name: CSAC-Bmal1
• gRNA/shRNA sequence: GGAACCGGAGAGTAGGTCGG, GCCTCTTTTCAATCTGACTG
• Species: M. musculus (mouse)
• Promoter: U6, hSyn
• Tag / Fusion Protein:
  • mCherry (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BsaI (not destroyed)
• 3′ cloning site: BsaI (not destroyed)
• 5′ sequencing primer: pUC_F ggagcctatggaaaaacgccag
• 3′ sequencing primer: hSyn_R gtcggtcgtcaggtaggcac

Resource Information

• Supplemental Documents:
  • paav-u6-bmal1-1-3-hsyn-mcherry-kash_bmal1-goldengate.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry

Depositor Comments

When comparing to the depositor reference sequence, please note that the mutations K14E, N210D, and E249G in mCherry-KASH do not alter plasmid function.