PB-3XpolyA-3XPC-miniCMV-mCherry-WPRE-insulator
(Plasmid
#168291)
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Purposeoptimized miniCMV (OminiCMV)
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 168291 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonePB
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namemCherry
- Promoter miniCMV
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer n/a (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
PB-3XpolyA-3XPC-miniCMV-mCherry-WPRE-insulator was a gift from Hui Yang (Addgene plasmid # 168291 ; http://n2t.net/addgene:168291 ; RRID:Addgene_168291) -
For your References section:
Endogenous promoter-driven sgRNA for monitoring the expression of low-abundance transcripts and lncRNAs. Gao N, Hu J, He B, Ji Z, Hu X, Huang J, Wei Y, Peng J, Wei Y, Zhou Y, Shen X, Li H, Feng X, Xiao Q, Shi L, Sun Y, Zhou C, Zhou H, Yang H. Nat Cell Biol. 2021 Jan;23(1):99-108. doi: 10.1038/s41556-020-00610-9. Epub 2021 Jan 4. 10.1038/s41556-020-00610-9 PubMed 33398178