pNIC-AncCAR_A
(Plasmid #168286)

• Purpose: Expresses AncCAR_A in E. coli from a T7 promoter
• Depositing Lab: Nicholas Harmer
• Publication: Thomas et al Commun Biol. 2019 Nov 22;2:429. doi: 10.1038/s42003-019-0677-y. eCollection 2019.

Backbone

• Vector backbone: pNIC28-Bsa4
• Backbone manufacturer: Structural Genomics Consortium
• Backbone size w/o insert (bp): 7284
• Total vector size (bp): 8775
• Modifications to backbone: Removal of sacB counterselection marker
• Vector type: Bacterial Expression, Synthetic Biology

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: AncCAR_A
• Species: Synthetic
• Insert Size (bp): 3460
• Promoter: T7
• Tag / Fusion Protein:
  • 6*His (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: MfeI (not destroyed)
• 3′ cloning site: HindIII (not destroyed)
• 5′ sequencing primer: Bespoke
• 3′ sequencing primer: T7

Resource Information

• Supplemental Documents:
  • pNIC-AncCAR_A.gb
• A portion of this plasmid was derived from a plasmid made by: N/A

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry