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Addgene

Bin1 N-BAR domain
(Plasmid #168280)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 168280 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pEGFP-N1
  • Backbone size w/o insert (bp) 4733
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Bin1
  • Alt name
    Bridging integrator 1
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    801
  • Mutation
    aa 1-267
  • GenBank ID
    NM_001083334.1
  • Entrez Gene
    Bin1 (a.k.a. ALP-1, Am, Amphl, BRAMP-2, SH3P9)
  • Tag / Fusion Protein
    • EGFP (C terminal on backbone)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Wildtype Bin1 plasmid was from Addgene 27693.

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Bin1 N-BAR domain was a gift from Cheng-han Yu (Addgene plasmid # 168280 ; http://n2t.net/addgene:168280 ; RRID:Addgene_168280)

  • For your References section:

    Podosome formation promotes plasma membrane invagination and integrin-beta3 endocytosis on a viscous RGD-membrane. Cao F, Zhou Y, Liu X, Yu CH. Commun Biol. 2020 Mar 13;3(1):117. doi: 10.1038/s42003-020-0843-2. 10.1038/s42003-020-0843-2 PubMed 32170110
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