DNM2 delta PRD
(Plasmid
#168275)
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PurposeExpress PRD-deleted DNM2 mutant (aa 1-742) in pEGFP-N1 vector.
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 168275 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepEGFP-N1
- Backbone size w/o insert (bp) 4722
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameDNM2
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Alt namedynamin 2
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SpeciesH. sapiens (human)
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Insert Size (bp)2226
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Mutationaa 1-742
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GenBank IDBC054501.1
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Entrez GeneDNM2 (a.k.a. CMT2M, CMTDI1, CMTDIB, DI-CMTB, DYN2, DYNII, LCCS5)
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Tag
/ Fusion Protein
- GFP (C terminal on backbone)
Resource Information
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A portion of this plasmid was derived from a plasmid made byWildtype DNM2 plasmid was from Addgene #27689.
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
DNM2 delta PRD was a gift from Cheng-han Yu (Addgene plasmid # 168275 ; http://n2t.net/addgene:168275 ; RRID:Addgene_168275) -
For your References section:
Podosome formation promotes plasma membrane invagination and integrin-beta3 endocytosis on a viscous RGD-membrane. Cao F, Zhou Y, Liu X, Yu CH. Commun Biol. 2020 Mar 13;3(1):117. doi: 10.1038/s42003-020-0843-2. 10.1038/s42003-020-0843-2 PubMed 32170110