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Addgene

LiPOP1-NanoLuc
(Plasmid #168265)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 168265 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    mCherry2-N1
  • Backbone manufacturer
    Addgene
  • Backbone size w/o insert (bp) 4750
  • Total vector size (bp) 7122
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    MLKL(1-125)
  • Species
    H. sapiens (human)
  • Mutation
    H15A, K16A, R17A
  • GenBank ID
    NM_152649.3
  • Entrez Gene
    MLKL (a.k.a. hMLKL)
  • Promoter CMV
  • Tag / Fusion Protein
    • mCherry, NanoLuc, CRY2-PHR (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NheI (not destroyed)
  • 3′ cloning site NheI (not destroyed)
  • 5′ sequencing primer CMV-For
  • 3′ sequencing primer Sv40pA-R
    • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    LiPOP1-NanoLuc was a gift from Yubin Zhou (Addgene plasmid # 168265 ; http://n2t.net/addgene:168265 ; RRID:Addgene_168265)

  • For your References section:

    Optogenetic Control of Non-Apoptotic Cell Death. He L, Huang Z, Huang K, Chen R, Nguyen NT, Wang R, Cai X, Huang Z, Siwko S, Walker JR, Han G, Zhou Y, Jing J. Adv Sci (Weinh). 2021 May 6;8(13):2100424. doi: 10.1002/advs.202100424. eCollection 2021 Jul. 10.1002/advs.202100424 PubMed 34540558
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