LiPOP1
(Plasmid #168264)

• Purpose: A single-component optogenetic tool for photoswitchable necroptosis
• Depositing Lab: Yubin Zhou
• Publication: He et al Adv Sci (Weinh). 2021 May 6;8(13):2100424. doi: 10.1002/advs.202100424. eCollection 2021 Jul.

Backbone

• Vector backbone: mCherry2-N1
• Backbone manufacturer: Addgene
• Backbone size w/o insert (bp): 4750
• Total vector size (bp): 6600
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: MLKL(1-125)
• Species: H. sapiens (human)
• Mutation: H15A, K16A, R17A
• GenBank ID: NM_152649.3
• Entrez Gene: MLKL (a.k.a. hMLKL)
• Promoter: CMV
• Tag / Fusion Protein:
  • mCherry, CRY2-PHR (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NheI (not destroyed)
• 3′ cloning site: NheI (not destroyed)
• 5′ sequencing primer: CMV-For
• 3′ sequencing primer: Sv40pA-R

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry