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Tol2-LyzC-RacFRET-U6ac-ctrl guides
(Plasmid #168247)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 168247 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pME-GFP
  • Modifications to backbone
    remove GFP and add RacFRET
  • Vector type
    Zebrafish expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    RacFRET
  • gRNA/shRNA sequence
    gggccaatgcgagaccctgt/gggctgtatcccagagtccc
  • Promoter LyzC

Cloning Information

  • Cloning method Gateway Cloning
  • 5′ sequencing primer GGTCGACTCGCCACCATGGTGAGCAAGGGCGAGG
  • 3′ sequencing primer CTCCACCGCGGCCGCTTACATAATTACACACTTTGTC
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Reference for the RacFRET (Raichu-Rac1) element:
D. L. Graham, P. N. Lowe, and P. A. Chalk. A method to measure the interaction of Rac/Cdc42 with their binding partners using fluorescence resonance energy transfer between mutants of green fluorescent protein. Anal.Biochem. 296:208-217, 2001.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Tol2-LyzC-RacFRET-U6ac-ctrl guides was a gift from Qing Deng (Addgene plasmid # 168247 ; http://n2t.net/addgene:168247 ; RRID:Addgene_168247)

  • For your References section:

    A robust and flexible CRISPR/Cas9-based system for neutrophil-specific gene inactivation in zebrafish. Wang Y, Hsu AY, Walton EM, Park SJ, Syahirah R, Wang T, Zhou W, Ding C, Lemke AP, Zhang G, Tobin DM, Deng Q. J Cell Sci. 2021 Apr 15;134(8). pii: 237799. doi: 10.1242/jcs.258574. Epub 2021 Apr 22. 10.1242/jcs.258574 PubMed 33722979
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