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XE224 human flag-Chibby-pCS3+FLT
(Plasmid #16824)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 16824 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCS3+FLT
  • Backbone manufacturer
    ATCC
  • Vector type
    Xenopus Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    flag-Chibby
  • Alt name
    cby
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1000
  • GenBank ID
    AL050345
  • Entrez Gene
    CBY1 (a.k.a. C22orf2, CBY, Chibby1, HS508I15A, PGEA1, PIGEA-14, PIGEA14, arb1)
  • Tag / Fusion Protein
    • flag (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoR1 (unknown if destroyed)
  • 3′ cloning site Xho1 (unknown if destroyed)
  • 5′ sequencing primer SP6
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The full coding sequence as well as 3' UTR of Human Chibby, ~1kb, was amplified by PCR and subcloned into the EcoR1 and Xho1 sites of pCS3+FLT, providing a flag tag.

Reference: K. Takemaru et al., Nature 422: 905 (2003)

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    XE224 human flag-Chibby-pCS3+FLT was a gift from Randall Moon (Addgene plasmid # 16824 ; http://n2t.net/addgene:16824 ; RRID:Addgene_16824)

  • For your References section:

    Chibby, a nuclear beta-catenin-associated antagonist of the Wnt/Wingless pathway. Takemaru K, Yamaguchi S, Lee YS, Zhang Y, Carthew RW, Moon RT. Nature. 2003 Apr 24. 422(6934):905-9. 10.1038/nature01570 PubMed 12712206
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