pMK433 (mAID-P2A-BSD)
(Plasmid
#168228)
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PurposeC-terminal tagging with mAID
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 168228 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepBluescript
- Total vector size (bp) 4125
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Vector typeMammalian Expression, CRISPR
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Selectable markersBlasticidin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namemAID-P2A-BSD
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SpeciesSynthetic
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pMK433 (mAID-P2A-BSD) was a gift from Masato Kanemaki (Addgene plasmid # 168228 ; http://n2t.net/addgene:168228 ; RRID:Addgene_168228) -
For your References section:
Targeted Protein Depletion Using the Auxin-Inducible Degron 2 (AID2) System. Saito Y, Kanemaki MT. Curr Protoc. 2021 Aug;1(8):e219. doi: 10.1002/cpz1.219. 10.1002/cpz1.219 PubMed 34370399