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Addgene

pMK432 (mAID-P2A-Hygro)
(Plasmid #168227)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 168227 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pBluescript
  • Total vector size (bp) 4821
  • Vector type
    Mammalian Expression, CRISPR
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    mAID-P2A-Hygro
  • Species
    Synthetic

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMK432 (mAID-P2A-Hygro) was a gift from Masato Kanemaki (Addgene plasmid # 168227 ; http://n2t.net/addgene:168227 ; RRID:Addgene_168227)
  • For your References section:

    Targeted Protein Depletion Using the Auxin-Inducible Degron 2 (AID2) System. Saito Y, Kanemaki MT. Curr Protoc. 2021 Aug;1(8):e219. doi: 10.1002/cpz1.219. 10.1002/cpz1.219 PubMed 34370399