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XE226 Drosophila Chibby-pCS2P+
(Plasmid #16822)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 16822 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCS2P+
  • Backbone size w/o insert (bp) 4100
  • Vector type
    Xenopus Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Drosophila Chibby
  • Alt name
    cby
  • Species
    D. melanogaster (fly)
  • Insert Size (bp)
    300
  • Entrez Gene
    Cby (a.k.a. Dmel_CG13415, CG13415, Dmel\CG13415)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamH1 (unknown if destroyed)
  • 3′ cloning site EcoR1 (unknown if destroyed)
  • 5′ sequencing primer NA
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Description: The full coding sequence of Drosophila Chibby, 0.3kb, was amplified by PCR and subcloned into the BamH1 and EcoR1 sites of pCS2P+.

Reference: K. Takemaru et al., Nature 422: 905 (2003)

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    XE226 Drosophila Chibby-pCS2P+ was a gift from Randall Moon (Addgene plasmid # 16822 ; http://n2t.net/addgene:16822 ; RRID:Addgene_16822)

  • For your References section:

    Chibby, a nuclear beta-catenin-associated antagonist of the Wnt/Wingless pathway. Takemaru K, Yamaguchi S, Lee YS, Zhang Y, Carthew RW, Moon RT. Nature. 2003 Apr 24. 422(6934):905-9. 10.1038/nature01570 PubMed 12712206
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