XE41 Rat frizzled-1-CS2+
(Plasmid
#16821)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 16821 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepCS2+
- Backbone size w/o insert (bp) 4095
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Vector typeXenopus Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namerat frizzled 1
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Alt nameFRD
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SpeciesR. norvegicus (rat)
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Insert Size (bp)2044
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Entrez GeneFzd1
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (unknown if destroyed)
- 5′ sequencing primer SP6 (Common Sequencing Primers)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Transcription: linearize with Not I or Asp 718 and transcribe with SP6.
Plasmid History:
Constructed by Julia Yang-Snyder on the date of 4/29/96.
Original reference: Chan, S.D.H., et al. JBC 267:25202-25207, 1992.
Published reference: Yang-Snyder, J.A. et al. Curr. Biol. 6:1302-1306, 1996.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
XE41 Rat frizzled-1-CS2+ was a gift from Randall Moon (Addgene plasmid # 16821 ; http://n2t.net/addgene:16821 ; RRID:Addgene_16821) -
For your References section:
A frizzled homolog functions in a vertebrate Wnt signaling pathway. Yang-Snyder J, Miller JR, Brown JD, Lai CJ, Moon RT. Curr Biol. 1996 Oct 1. 6(10):1302-6. 10.1016/S0960-9822(02)70716-1 PubMed 8939578