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pCS2-zfCSF3a
(Plasmid #168201)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 168201 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCS2+
  • Backbone size w/o insert (bp) 4095
  • Total vector size (bp) 4790
  • Vector type
    Mammalian Expression ; Vertebrate expression (zebrafish, chicken, xenopus)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Csf3a
  • Alt name
    Gcsf3a
  • Species
    D. rerio (zebrafish)
  • Insert Size (bp)
    686
  • Mutation
    Deletion of A27
  • GenBank ID
    NM_001145242
  • Entrez Gene
    csf3a (a.k.a. csf3, gcsf)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site XbaI (not destroyed)
  • 5′ sequencing primer SP6
  • 3′ sequencing primer T7
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please note deletion of A27 in the insert is a common SNP and does not affect function.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCS2-zfCSF3a was a gift from Petr Bartunek (Addgene plasmid # 168201 ; http://n2t.net/addgene:168201 ; RRID:Addgene_168201)

  • For your References section:

    The zebrafish granulocyte colony-stimulating factors (Gcsfs): 2 paralogous cytokines and their roles in hematopoietic development and maintenance. Stachura DL, Svoboda O, Campbell CA, Espin-Palazon R, Lau RP, Zon LI, Bartunek P, Traver D. Blood. 2013 Dec 5;122(24):3918-28. doi: 10.1182/blood-2012-12-475392. Epub 2013 Oct 15. 10.1182/blood-2012-12-475392 PubMed 24128862
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